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r2c rat leydig tumor cells  (ATCC)


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    ATCC r2c rat leydig tumor cells
    R2c Rat Leydig Tumor Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+leydig+tumor+cells+r2c/pm38944211-48-0-12?v=ATCC
    Average 94 stars, based on 126 article reviews
    r2c rat leydig tumor cells - by Bioz Stars, 2026-07
    94/100 stars

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    94
    ATCC r2c rat leydig tumor cells
    R2c Rat Leydig Tumor Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+leydig+tumor+cells+r2c/pm38944211-48-0-12?v=ATCC
    Average 94 stars, based on 1 article reviews
    r2c rat leydig tumor cells - by Bioz Stars, 2026-07
    94/100 stars
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    90
    ATCC rat leydig cell tumor cells
    Cholesterol trafficking analysis in primary <t>rat</t> <t>Leydig</t> cells infected with lentivirus containing mCherry-D4. A, confocal microscope images of cells with and without addition of Bt2cAMP. Scale bar, 10 μm. B, time course of mCherry-D4 fluorescence intensity at the plasma membrane of control and Bt2cAMP-treated cells. C, testosterone content of control cells and cells infected with lentivirus before and after a 2-h treatment with Bt2cAMP. Testosterone was measured just after the cells were isolated (0 h), in control (uninfected) cells 18 h after incubation and 18 h after infection. Data represent means ± S.D. of at least three independent experiments performed in triplicate; two-way ANOVA followed by Bonferroni's post hoc test (*) was used to calculate statistical significance. *, p < 0.05; **, p < 0.01; ***, p < 0.001. dbcAMP, dibutyryl cAMP.
    Rat Leydig Cell Tumor Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+leydig+tumor+cells+r2c/pmc05207080-915-22-27?v=ATCC
    Average 90 stars, based on 1 article reviews
    rat leydig cell tumor cells - by Bioz Stars, 2026-07
    90/100 stars
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    94
    ATCC rat leydig tumor cells r2c
    Cholesterol trafficking analysis in primary <t>rat</t> <t>Leydig</t> cells infected with lentivirus containing mCherry-D4. A, confocal microscope images of cells with and without addition of Bt2cAMP. Scale bar, 10 μm. B, time course of mCherry-D4 fluorescence intensity at the plasma membrane of control and Bt2cAMP-treated cells. C, testosterone content of control cells and cells infected with lentivirus before and after a 2-h treatment with Bt2cAMP. Testosterone was measured just after the cells were isolated (0 h), in control (uninfected) cells 18 h after incubation and 18 h after infection. Data represent means ± S.D. of at least three independent experiments performed in triplicate; two-way ANOVA followed by Bonferroni's post hoc test (*) was used to calculate statistical significance. *, p < 0.05; **, p < 0.01; ***, p < 0.001. dbcAMP, dibutyryl cAMP.
    Rat Leydig Tumor Cells R2c, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+leydig+tumor+cells+r2c/pm33460247-71-9-17?v=ATCC
    Average 94 stars, based on 1 article reviews
    rat leydig tumor cells r2c - by Bioz Stars, 2026-07
    94/100 stars
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    94
    ATCC r2c rat leydig tumor cell line
    Cholesterol trafficking analysis in primary <t>rat</t> <t>Leydig</t> cells infected with lentivirus containing mCherry-D4. A, confocal microscope images of cells with and without addition of Bt2cAMP. Scale bar, 10 μm. B, time course of mCherry-D4 fluorescence intensity at the plasma membrane of control and Bt2cAMP-treated cells. C, testosterone content of control cells and cells infected with lentivirus before and after a 2-h treatment with Bt2cAMP. Testosterone was measured just after the cells were isolated (0 h), in control (uninfected) cells 18 h after incubation and 18 h after infection. Data represent means ± S.D. of at least three independent experiments performed in triplicate; two-way ANOVA followed by Bonferroni's post hoc test (*) was used to calculate statistical significance. *, p < 0.05; **, p < 0.01; ***, p < 0.001. dbcAMP, dibutyryl cAMP.
    R2c Rat Leydig Tumor Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+leydig+tumor+cells+r2c/10__1530_slash_jme___19___0063-66-1-10?v=ATCC
    Average 94 stars, based on 1 article reviews
    r2c rat leydig tumor cell line - by Bioz Stars, 2026-07
    94/100 stars
      Buy from Supplier

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    Cholesterol trafficking analysis in primary rat Leydig cells infected with lentivirus containing mCherry-D4. A, confocal microscope images of cells with and without addition of Bt2cAMP. Scale bar, 10 μm. B, time course of mCherry-D4 fluorescence intensity at the plasma membrane of control and Bt2cAMP-treated cells. C, testosterone content of control cells and cells infected with lentivirus before and after a 2-h treatment with Bt2cAMP. Testosterone was measured just after the cells were isolated (0 h), in control (uninfected) cells 18 h after incubation and 18 h after infection. Data represent means ± S.D. of at least three independent experiments performed in triplicate; two-way ANOVA followed by Bonferroni's post hoc test (*) was used to calculate statistical significance. *, p < 0.05; **, p < 0.01; ***, p < 0.001. dbcAMP, dibutyryl cAMP.

    Journal: The Journal of Biological Chemistry

    Article Title: Plasma Membrane Origin of the Steroidogenic Pool of Cholesterol Used in Hormone-induced Acute Steroid Formation in Leydig Cells *

    doi: 10.1074/jbc.M116.740928

    Figure Lengend Snippet: Cholesterol trafficking analysis in primary rat Leydig cells infected with lentivirus containing mCherry-D4. A, confocal microscope images of cells with and without addition of Bt2cAMP. Scale bar, 10 μm. B, time course of mCherry-D4 fluorescence intensity at the plasma membrane of control and Bt2cAMP-treated cells. C, testosterone content of control cells and cells infected with lentivirus before and after a 2-h treatment with Bt2cAMP. Testosterone was measured just after the cells were isolated (0 h), in control (uninfected) cells 18 h after incubation and 18 h after infection. Data represent means ± S.D. of at least three independent experiments performed in triplicate; two-way ANOVA followed by Bonferroni's post hoc test (*) was used to calculate statistical significance. *, p < 0.05; **, p < 0.01; ***, p < 0.001. dbcAMP, dibutyryl cAMP.

    Article Snippet: MA-10 cells from mouse Leydig cell tumor (kindly provided by Dr. M. Ascoli, University of Iowa, Iowa City) and R2C cells from rat Leydig cell tumor cells (American Type Culture Collection, Manassas, VA) were cultured with Dulbecco's modified Eagle's medium (DMEM)/Ham's F-12 (Invitrogen) supplemented with 1% penicillin and 1% streptomycin and maintained at 37 °C.

    Techniques: Infection, Microscopy, Fluorescence, Isolation, Incubation